Quick Overview
Sample: Bacterial Culture
Yield: Up to 200 µg Plasmid DNA
25 Prep
Protocol TIme: ~1 Hour
Yield: Up to 200 µg Plasmid DNA
25 Prep
Protocol TIme: ~1 Hour
Details
White Box Plasmid Midi Kit:
- Isolate up to 200 µg of plasmid DNA from bacterial culture samples
- Can utilize up to 100 mL of bacterial culture
- Quick and efficient spin column protocol utilizing alkaline SDS lysis (~1 hour)
- No phenol/chloroform extraction needed
- Purified DNA is suitable for downstream applications
- Copy number varies with plasmid size (5 to 700 Copies)
- Compatible with vacuum manifolds
Kit Contents:
25x DNA Midi Columns
25x 15 mL Collection Tubes
1x 70 mL Solution I
1x 70 mL Solution II
1x 100 mL Solution III
1x 58 mL HBC Buffer
1x 40 mL DNA Wash Buffer
1x 60 mL Elution Buffer
1x 300 µL RNase A
1x User Manual
- Isolate up to 200 µg of plasmid DNA from bacterial culture samples
- Can utilize up to 100 mL of bacterial culture
- Quick and efficient spin column protocol utilizing alkaline SDS lysis (~1 hour)
- No phenol/chloroform extraction needed
- Purified DNA is suitable for downstream applications
- Copy number varies with plasmid size (5 to 700 Copies)
- Compatible with vacuum manifolds
Kit Contents:
25x DNA Midi Columns
25x 15 mL Collection Tubes
1x 70 mL Solution I
1x 70 mL Solution II
1x 100 mL Solution III
1x 58 mL HBC Buffer
1x 40 mL DNA Wash Buffer
1x 60 mL Elution Buffer
1x 300 µL RNase A
1x User Manual
Additional Information
| UNSPSC | No |
|---|---|
| Applications | Plasmid Isolation (Midiprep) |
| Nucleic Acid Binding Capacity | No |
| Nucleic Acid | Plasmid |
| Number of Preps | 25 |
| Protocol Time | 1 Hour |
| Sample Type | Bacterial Culture |
| Target Length | N/A |
| Yield | Up To 200 µg Plasmid DNA |
